This Cas9 nuclease can be used with a target-specific sgRNA to bind DNA and create a double-stranded cut. It is provided in a non-viral vector (plasmid) for quick and easy transfection. Transfection will result in transient expression of Cas9, leading to reduced chances of off-target editing. The Cas9 provided in this non-viral vector is spCas9 from Streptococcus pyogenes.
Applications
- Direct non-viral plasmid transfection for immediate expression.
- Transient sgRNA expression reduces the chance of off-target effects.
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